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KMID : 0616120110420030246
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Korean Journal of Pharmacognosy
2011 Volume.42 No. 3 p.246 ~ p.252
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Immunoadjuvant Activity of Korean Mistletoe Lectin B-chain
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Her Sun-Mi
An Hyo-Sun
Kim Young-Hoon
Yoon Taek-Joon
Kim Jong-Bae
Kim Kyu-Dae
Kim In-Bo
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Abstract
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Korean mistletoe Lectin (KML-C) is composed of A and B sub-chain. B chain binds to carbohydrates on cell surface and A chain hinders translation and induces an apoptosis as a RIP (ribosome inactivating protein). KML-C has very strong biological activities, it has seriously limits to use as a cancer therapy or adjuvant because of its toxicity to normal cells. This study is therefore conducted to see if B chain of KML-C might have immunological activity, especially adjuvant activities with less toxicity. We isolated B chain from KML-C using the lactose affinity chromatography, and examined their immunoadjuvant activity. The isolated B-chain did not show any cytotoxicity against tumor cell, RAW264.7, and P388D1 while KML-C had a very strong toxicity. This non-toxic effect was observed also by in-vivo study. Both humoral and cellular immunities were observed ; the antibody titer was increased when the mice were immunized with B-chain used as adjuvant like Freund’s adjuvant, indicating that B chain of mistletoe lectin alone might be used for adjuvant; it also increased DTH in cellular immunity. These results suggest that B-chain of KML-C might be used for adjuvant used for the production of antibody or vaccine with less toxicity
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KEYWORD
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Korean mistletoe lectin (KML-C), B chain, Immunoadjuvant, Antibody, DTH
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